脱细胞真皮移植部位血管细胞黏附分子1的表达

EXPRESSION OF VASCULAR CELL ADHESION MOLECULE 1 IN ACELLULAR DERMAL MATRIX GRAFTING IN PIGS

目的探讨血管细胞黏附分子1(vascular cell adhesion molecule 1,VCAM-1)在脱细胞真皮移植部位的表达变化和意义。方法取近交系小型香猪15只建立猪实验模型,分别于每只猪脊背两侧制作6cm×6cm各3处6个创面。随机分为三组,每组5只。A组为对照组,猪刃厚自体皮移植;B组异体脱细胞真皮与猪刃厚自体皮复合移植;C组J-1型(人)脱细胞真皮与猪刃厚皮复合移植。分别于移植术后3、9、21及30d观察移植皮片成活情况和面积,于移植后3、6、9、12、21及30d各时间点取移植组织标本,流式细胞仪检测VCAM-1的表达变化。结果移植术后3d各组移植皮片基底分离;9dA组皮片转红润基底粘连较紧密,揭开皮片可见渗血,B、C组皮片颜色次之,基底粘连不紧密;21d,A、B、C组移植皮片成活面积分别为94%±12%、92%±9%和91%±11%;术后30d各组移植皮片均愈合良好。各组VCAM-1表达,移植术后3d无明显差别;6dA组表达明显高于B、C组有统计学意义(P<0.05);9、12dB、C组明显高于A组有统计学意义(P<0.05);30d,各组VCAM-1表达均低于3d有统计学意义(P<0.01)。结论同种和异种真皮基质移植部位VCAM-1表达高峰迟滞于自体皮移植;提示VCAM-1表达可能与脱细胞真皮活化、血管再生有关。同种和异种真皮移植物VCAM-1表达无差别。

Objective To explore the expression of the vascular cell adhesion molecule 1 （VCAM-1） in the aeellular dermal matrix grafting in pigs. Methods Experimental models were established with 15 Inbred Strain mini pigs, 6 full-thiehness skin defect wounds, 6 cm × 6 cm in size, were produced on both-side backs of the each pig, and then the pigs were randomly divided into 3 groups. In Group A （n： 5, control） the thin auto-skin transplantation alone was made ; in Group B （n= 5）, the grafting was performed in the aeellular allo-dermal matrix combined with the thin auto-skins; in Group C （n=5）, the grafting was performed in the aeellular xeno-dermal matrix combined with the thin auto-skins. The areas of the wounds were measured and the survival condition of the grafted skins was observed at 3, 9, 21 and 30 days after the grafting. The histological samples were harvested from the grafting area at 3, 6, 9, 12, 21 and 30 days after the procedure. The flow eytometry was employed to analyze the changes in the VCAM-1 level in the sample at different time points after the grafting. Results In the 3 groups, the transplanted skin base was easily separated at 3 days after transplantation; the areas of the wound healing accounted for 94%± 12%, 92%±9%, and 91%±11%, respectively, at 21 days; good wound healing was achieved at 30 days. At 9 and 12 days after transplantation, there was an evidently-increased level of the VCAM-1 expression in the tissue samples in the composite skin grafting groups. Compared with the control group, the difference was significant （P 〈 0. 05）; however, the VCAM-1 expression at 3 days was not statistically different between the composite skin grafting groups and the control group after transplantation. In contrast, the level of the VCAM-1 expression was significantly higher at 6 days in the control group than in the composite skin grafting groups （P〈0.05）. The levels of the VCAM-1 expression were significantly lower at 30 days than at 3 days after transplantation in all the 3 groups （P〈0. 01）. Conclusion The highest level of the VCAM-1 expression can be delayed in the composite skin grafting when compared with that in the thin auto-skins alone, which implies that the VCAM-1 expression may be correlated with angiogenesis and composite skin survival. The VCAM-1 expression is not different between the aeellular allo-dermal matrix composite skin grafting groups and the aeellular xeno-dermal matrix group.