糖尿病仓鼠视网膜p38MAPK和TGFβ_2的表达

Expression of p38MAPK and TGFβ_2 in retina of experimental diabetic hamster

目的探讨p38MAPK、TGFβ2在糖尿病视网膜病变(diabetic retinopathy,DR)发生发展中的作用及其机制。方法用链脲佐菌素(streptozotocin,STZ)诱导仓鼠糖尿病(DM)模型,提取视网膜中总RNA,半定量RT-PCR观察视网膜中p38MAPK、TGFβ2 mRNA表达情况,Western blotting检测DM仓鼠p38MAPK、TGFβ2蛋白情况。结果DM仓鼠视网膜中p38MAPK、TGFβ2 mRNA表达呈显著升高,蛋白表达增多。结论p38MAPK信号途径参与DR的发病机,制阻断p38MAPK的活性可能有助于阻断DR的发生和发展。

Objective To explore the role of p38MAPK and TGFβ2 in the development of diabetic retinopathy. Methods Fifteen Hamsters were induced into diabetic models by intraperitoneal injection of Streptozotocin （40 mg/kg once a day） for 3 d and 13 Hamsters were successfully established, whose blood glucose was over 13.5 mmol/L. Ten Hamsters as controls were intraperitoneally injected of physical saline of the same volume. At 16^th week after induction, the total RNA of retina from all sacrificed Hamsters was collected. The mRNA expressions of p38MAPK,TGFβ2 in retina were detected by semi-quantitative RT-PCR and their protein levels by Western blotting. Results The mRNA expressions of p38MAPK, TGFβ2 in retina were of high tendency and their protein levels increased. Conclusion p38MAPK signal pathway may involve in the pathogenesis of diabetic retinopathy.