胰岛素基因增强结合蛋白1基因修饰的神经干细胞向胆碱能神经元分化的实验研究

THE STUDY OF THE DIFFERENTIATION OF NSCs MODIFIED WITH ISLET-1 GENE INTO CHOLINERGIC NEURONS

目的探讨大鼠胰岛素基因增强结合蛋白1(Islet-1)基因是否有诱导神经干细胞(NSCs)向胆碱能运动神经元分化的能力。方法用Islet-1基因重组逆转录病毒载体转导NSCs后,用免疫荧光组织化学染色方法检测Islet-1在NSCs内的表达;体内、体外实验观察导入Islet-1基因的NSCs向乙酰胆碱转移酶(ChAT)阳性细胞分化的情况。结果在体外分化实验中观察到,转导Islet-1基因的NSCs向胆碱能运动神经元分化的细胞数明显多于对照组;体内移植实验发现,导入Islet-1基因的NSCs在体内可以向ChAT阳性细胞分化。结论Islet-1基因有诱导NSCs向胆碱能运动神经元分化的能力。

Objective To explore whether Islet-1 gene of the rat could induce NSCs to differentiate into cholinergic neurons. Methods NSCs were transduced with Islet-1 recombinant retroviral expression vector. The protein expression of Islet-1 gene in NSCs was detected by immunofluorescence histochemical method. The ability of NSCs to differentiate into ChAT positive cells was observed in vivo and in vitro. Results The numbers of ChAT positive cells were significantly increased in the group of NSCs modified with Islet-1 compared with the control group in vitro. NSCs modified with Islet-1 could differentiate into ChAT positive cells when they were grafted into the corpus striattun of the adult rat brain. Conclusion Islet-1 gene could induce NSCs to differentiate into cholinergic neurons.