人类RUNX3基因对胃癌分化及预后影响的再评价

Reappraisal of the impacts of human Rant-related transcription factor gene 3 expression on differentiation and prognosis of gastric cancer

目的研究人类Runt相关转录因子基因3（RUNX3）蛋白在胃癌组织中的表达特征，探讨该蛋白表达与胃癌分化类型等临床病理特征及其预后的关系。方法用免疫组织化学方法（免疫组化）和Western印迹技术，检测66例胃癌原发灶组织中RUNX3蛋白的表达，并研究该蛋白表达与患者长期生存率的关系。结果（1）免疫组化染色结果提示，所测胃癌组织中，RUNX3蛋白表达阳性率为60．6％（40／66），且阳性颗粒定位于细胞浆：Western印迹结果显示，RUNX3蛋白在肿瘤中的表达明显高于癌旁非肿瘤组织；（2）RUNX3蛋白表达与肿瘤组织分化程度（P=0．025）和Lauren分型（P=0．034）密切相关，而与TNM分期无关（P=0．085）；（3）肿瘤组织RUNX3蛋白表达和无表达组胃癌患者的中位生存期分别为2187d和2478d，差异有统计学意义（P=0．016）。结论RUNX3表达异常可影响胃癌分化，且RUNX3表达与患者预后呈明显负相关关系。该基因作为典型抑癌基因的价值，及其在胃癌发生发展不同阶段中所发挥的作用有待进一步探讨。

Objective To investigate the expression of Runt-related transcription factor gene 3 （RUNX3） in gastric cancer and its impact on the outcome of gastric cancer patients. Methods By using immunohistochemistry staining and western blot assay, the expression of RUNX3 protein in 66 cases of gastric cancer with various clinicopathologic characteristics were detected, and the effects of RUNX3 protein expression on the outcome of patients undergone surgical resection were evaluated. Results （1）The expression rate of RUNX3 protein in gastric cancer lesions was 60.6%（40/66）, and RUNX3 protein was mainly expressed in the cytoplasm of cancer ceils. RUNX3 protein expression in tumor tissues was significantly higher than that in non-tumor tissues. （2）RUNX3 protein expression was corelated with tumor differentiation （P=0.025） and Lauren＇s classification （P=0.034）, but had no relationship with the TNM stage（P=0.085）. （3）In sharp contrast, the median survival time of patients who had tumors with negative and positive RUNX3 protein expression were 2478 and 2187 days respectively（P=0.016）. Conclusions RUNX3 protein influences the differentiation of gastric cancer. The role of RUNX3 protein as a tumor-suppressor in tumorigenesis and differentiation of gastric carcinoma need to be further evaluated.