鼻咽癌中P＿（53）蛋白表达的研究

Immunohistochemicla study of P53 expression in nasopharyngeal carcinoma

本组用免疫组化ＳＰ法观察１１９例鼻咽癌（ＮＰＣ）中Ｐ＿（５３）蛋白表达，半定量研究发现：①Ｐ＿（５３）表达阳性率为５５．５％（６６／１１９），表达的阳性细胞强度和阳性细胞数量与ＮＰＣ的恶性度、组织学类型及淋巴结转移相关（Ｐ＜０．００５），尤其在未分化癌中有超表达现象。②在正常柱状、鳞状上皮，粘膜慢性炎症伴有单纯性增生者均阴性。而在不典型增生的鳞状上皮中有数量不等的轻或中度阳性表达，提示其基因突变趋向。③Ｐ＿（５３）表达阴性和阳性者平均生存期为６４．５和３１．１个月，两组间有显著性差异（Ｐ＜０．０５）。结果提示，检测ＮＰＣ中Ｐ＿（５３）蛋白表达，可作为了解其生物学特性及预测预后的指标之一。

Abstract P53 expression in 119 cases of nasopharyngeal carcioma(NPC)and19 cases of nasopha- ryngeal mucosa obtained from non-cancer patients were studied by SP immunohistOchemical methods,The results showed that:1).P53positive rate was 55.5%(66/119)in NPC,the cellular staining intensity of P53 expression was related to malignant level,histologicaI typing and metastatic lymph node(P<0. 005).Undifferentiated carcinoma had over-expression of P53 protein. 2).P53 expression was negative in normal columnar epithelia,squamous epithelia,arid chronic nasopharyngitis-epithelia with hyperplasia,but P53 expression was positive in atypical dysplasia,3).The survival duration was 64.5 and 31.1 months in P53 expression(-)and(+) patients,respectively(P<0.005).We belleve that examination of P53 protein was helpful to understand the biological behavior and estimate the prognosis.