藏绵羊生长激素释放激素基因内含子Ⅱ部分序列的分析

Analysis of Sequence of Intron Ⅱ of GHRH Gene on the Tibetan Sheep

对藏绵羊生长激素释放激素(GHRH)基因内含子Ⅱ部分序列进行了PCR扩增和测序,用Genbank中的Blastn方法与普通牛相应基因序列进行了比对分析。结果表明,藏绵羊与普通牛GHRH基因内含子Ⅱ部分序列间同源性为92%;2个物种该基因内含子Ⅱ部分序列间的碱基变异类型主要表现为碱基转换和颠换,亦存在碱基插入和缺失。

The intron Ⅱ of growth hormone releasing hormone gene of Tibetan sheep was amplified by PCR and sequenced. And the sequence was compared with that of cattle through Blastn method of Genebank. The results showed that the homology of nucleotide sequence of the intron Ⅱ of growth hormone releasing hormone gene between the Tibetan slfeep and the cattle was up to 92% and the chief base changes were basa transition,base transversion,base invertion and base deletion.