脐带血T淋巴细胞集落培养和亚群的关系

The Relationship of T Lymphocyte Colony Formation and T Cell Subset in Human Cord Blood

应用细胞培养和间接免疫荧光法测定脐带血、成人外周血、成人骨髓的Ｔ淋巴细胞集落形成情况及培养前后淋巴细胞亚群的变化。结果表明：脐带血的ＣＦＵ－ＴＬ显著低于成人外周血（Ｐ＜０．０１），略低于成人骨髓（Ｐ＜０．０５）；培养前脐带血的ＣＯ＋３、ＣＯ＋４细胞含量均显著低于成人外周血（Ｐ＜０．０１），与成人骨髓相近似。脐带血的ＣＤ＋８细胞含量与成人外周血和成人骨髓相似（Ｐ＜０．０５）；培养后的ＣＦＵ－ＴＬ中ＣＤ＋３细胞含量脐带血与成人外周血、成人骨髓几乎相同（Ｐ＞０．０５），但其中ＣＤ＋４细胞含量脐带血明显低于成人外周血及成人骨髓（Ｐ＜０．０１），而脐带血的ＣＤ＋８则明显高于成人外周血（Ｐ＜０．０１）和成人骨髓（Ｐ＜０．０５），致使培养后ＣＦＵ－ＴＬ中脐血ＣＤ＋４／ＣＤ＋８显著低于成人外周血和成人骨髓。另培养前的脐带血，成人骨髓中ＣＤ＋４、ＣＤ＋８细胞阳性百分率之和超过ＣＤ＋３百分率，出现ＣＤ＋４。

T lymphocyte colony form ation of human cord blood, adult peripheral blood (PB) and adult bone marrow (BM) was studied by means of cell culture and change in per-centage of T lymphocyte subsets before and after culture. The results showed that the number of CFU-TL of the cord blood was much lower than that of the normal PB(P<0.001) and slightly lower than of BM (P<0.05). The percentages of CD + 3 and CD + 4 cells in the cord blood before culture were much lower than those of normal PB (P<0.01), but was approximated to those of BM (P>0.05). The percentage of CD + 8 cells in the cord bllod was similar to that of the normal PB and BM (P>0.05) both. No significant difference was found in the percentage of CD + 3 cells in CFU-TL from the cord blood before culture and those of the normal PB and MB, but the per-centage of CD + 4 cells in cord blood was much lower than that either of the normal PB or BM (P<0.05) on the contrary the percentage of CD + 8 cells in the cord blood after culture was much higher than that form both the normal PB (P<0.01) and BM (P<0.05), thus, the ratio of CD + 4/CD + 8 in the cord blood after culture was much lower than that of either the adult PB or BM. In adclitron, the total percentage of CD + 4 and CD + 8 cells in cord blood or in BM before culture was higher than that of CD + 3 cells, This might be due to double labeling of the cells.