阳离子交换法分离纯化酵母细胞提取液中的谷胱甘肽

Glutathione Isolation and Purification of Yeast Extract with Ion Exchange Resin Columns

谷胱甘肽是重要的生物活性物质,本文采用国产D001阳离子交换双柱(A柱和B柱)对酵母细胞提取液中的GSH进行了分离纯化,确定了A、B离子交换柱分离纯化酵母提取液中的谷胱甘肽的工艺参数为:A柱上样的提取液pH为5．5～6．0;B柱上样液(A柱的收集液)的pH为2．4～2．8;B柱的洗脱采用1．2%的NaCl溶液。A、B两柱上样和洗脱液的流速均为0．5ml/min。酵母提取液中GSH的回收率大于70%,纯化倍数大于100倍。

Glutathione, one of the most important biological active substance plays a central role in metabolic pathways of live organisms. In this paper, A, B two cation ion-exchange columns were used to isolate and purify of GSH from yeast extract. The technology parameters of both A and B were： for column A, sample pH 5.5 -6.0, for column B, sample pH 2.6 -2.8, column B eluate 1.2% NaCl. The recovery rate of GSH is more than 70%, while the GSH has been purified more than 100 times.