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微波-酶法制备RS_3型玉米抗性淀粉工艺参数优化研究 被引量:20

Optimization of Parameters for Preparation of Corn Retrograded Starch by Microwave-Enzyme Method
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摘要 运用响应面法,对微波-酶法制备RS3型玉米抗性淀粉的工艺参数进行优化。α-淀粉酶酶解的优化工艺参数为:液料比4:1,酶解温度85℃,酶解时间10min,酶浓度1.68U/g淀粉:微波糊化的优化工艺参数为:功率1.26kW,加热温度92℃,加热时间1min:普鲁兰酶脱支优化工艺参数为:酶浓度4.13 NPUN/g淀粉,酶解温度53.31℃,酶解时间3.26h。按以上工艺参数制备的抗性淀粉得率为13.45%。 The processing parameters of microwave-enzyme method, used to produce corn retrograded starch, were optimized by respond surface methodology. The optimal hydrolyzing parameters by α-amylase are 85 ℃ for 10 min, the ratio of liquid to solid 4:1, α-amylase 1.68 U/g starch; the optimal pasting parameters with microwave are 1.26 kW 92 ℃ for lmin; the optimal dealing parameters with pullulanase are 53.31 ℃ for 3.26 h, pullulanase 4.13 NPUN/g starch. The productivity of resistant starch reaches 13.45% with the optimal processing method.
出处 《食品科学》 EI CAS CSCD 北大核心 2007年第4期237-240,共4页 Food Science
关键词 抗性淀粉 微波.酶法 响应面泫 Box-Behnken实验设计 resistant starch microwave-enzyme method response surface methodology (RSM) Box-Behnken design
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