人工基膜对鼻咽癌细胞株体外诱导分化的影响

Effect of Artificial Basement Membrane on the Differentiation of Nasopharyngeal Carcinoma Cell Line (CNE-2)

用全反维甲酸（ＲＡ）１０－７ｍｏｌ／Ｌ对生长在玻璃皿（Ｇ）和人工基膜（ＡＢＭ）上的鼻咽癌细胞株（ＣＮＥ－对细胞进行９ｄ的诱导分化。结果显示生长在Ｇ上的癌细胞增殖被抑制，软琼脂内集落形成和裸鼠体内生长等恶性表型减弱，生长在ＡＢＭ的细胞则不受影响，但其表面微绒毛丰富，细胞间的紧密连接、交指状连接、桥粒和腺腔样结构多见，高尔基器，粗面内浆网发育较好。表明不同的培养基质对ＲＡ的生物学作用有不同的影响，ＡＢＭ有拮抗ＲＡ抑制癌细胞增殖和去恶化的作用，但有助于癌细胞间结构的重建和细胞器的发育，提示在体外进行癌细胞诱导分化时应在近似体内环境的培养系统中进行，才能更确切地评价分化诱导剂的体内治疗反应。

Abstract The differentiation of CNE--2 Cells separately grown on the glass dishes (G) and on the artificial basement memdrane (ABM) induce by all--trans--retinoid acid 10--7mol/L was condu cted over nine days. TIP results showed that the inhibited prolifelation, the reduction of the soft agar--dependent anchorage and the transplantation in nude mice occurred in the cells grown on G but not on ABM, However, the Golgi 's body, the rough endoplasmic reticulum (RER), the cell surface microvilli , intercelular junctions such as tight junction, interdigitation, desmosome and acinic cavity construction were noted much more in the ABM cells than that in G. These findings suggested. 1) The ABM antagonized the inhibition role of RA on the cell proliferation and the reduction of malignant phenotypes, but helped the RA with the radonstruction of intercellular structure 2) The research work on differentiation of cancerous cells induced in vitro should. be made under the cultUlal system system mimetic to the enviroment in vivo so that the evaluation of the medicinal effects of agents inducing the differentiation in vivo will be more reliable .Keyworks: nasopharyngeal carcinoma, artificial basement membrane; retinoid acid, differentiation