转录因子GATA—1基因在白血病中的表达

The Expression of Transcription Factor GATA- 1 in Leukemia

转录因子ＧＡＴＡ家族在造血细胞的正常发育中起着重要的作用。利用聚合酶链反应（ＰＣＲ）方法分析了１１６例各种白血病中红系特异转录因子ＧＡＴＡ—１的表达情况。ＡＮＬＬ、ＣＭＬ、Ｃ－ＡＬＬ和ＣＬＬ中的表达率分别为４３．７５％、８８．２４％、１４．２９％和３３．３３％；３例Ｔ－ＡＬＬ均不表达该基因。从８０例ＡＮＬＬ中发现，ＭＯ、Ｍ１和Ｍ２亚型的ＧＡＴＡ－１回表达率较高，而３例Ｍ３型及ＨＬ６０细胞侏均不表达该基因。结果提示ＧＡＴＡ—１基因在多种白血病中的异常表达情况，它在粒单系白血病细胞的持续表达现象，可能与在干细胞或粒单系祖细胞水平的异常增殖、分化和成熟障碍并致白血病有关。

Transcription factor GATA family play an important role in the normal developmental processof hematopoietic cells . Using polymerase chain reaction (PCR) method the expression of erythroid specific transcription factor GATA-- 1 gene in 116 cases of leukemin was analyses. Expression later of GATA-- 1 gene in ANLL, CML, C--ALL and CLL were 43.75%, 88.24%, 14.29%and 33.33%, respectively, but no expression round in 3 cases of T--ALL. Highly expression of GATA-- 1 in MO, M1 and M2 subtype of 80 cases with ANLL was found. Bat completely negative expression was seen in 3 cases with M3 and HL60 (M3 cell line). The data show the phenomenon of GATA-- 1 gene aberrant expression in leukemia and suggest that GATA-- 1 gene continuous expression in granulocytic and monocytic leukemia cells mas be relate to abnormal proliferation and a block in the nounal pattern of differentiation andmaturation at the level of hematopoietic stem cell or early myeloid progenitor that result in leukermia.