口腔鳞癌体外药敏实验

In vitro drug-sensitivity test of primary culture of oral maxillofacial squamous cell carcinoma

目的寻找适宜应用于临床的体外敏感药物实验方法。方法对37例经病理确诊的口腔癌患者的口腔颌面部原代鳞状细胞癌,利用卡铂、5-氟尿嘧啶、平阳霉素、氨甲喋呤进行MTT法和SRB法药物敏感实验。结果①MTT法与SRB法所得结果差异无显著性(P>0.05)。②各药物的平均抑癌率依次为卡铂+5-氟尿嘧啶>卡铂>5-氟尿嘧啶>平阳霉素>氨甲喋呤。结论①MTT法与SRB法适合临床需要。②口腔鳞癌的药物敏感性与药物有关。③联合用药效果比单药效果好。

Objective To search a method of in vitro drug-sensitivity test which can be used in clinic. Methods The drug-sensitivity in 37 cases of primary culture of oral maxillofacial squamous cell carcinoma from the patients diagnosed pathologically as oral cancer were determined by MTT assay and SRB assay. Results ①There was no difference in drug-sensitivity between MTT assay and SRB assay（P〉0.05）.②The drug inhibitory effect was CBDCA＋ 5-Fu 〉 CBDCA 〉 5-Fu 〉 PYM 〉 MTX. Conclusion ①MTT assay and SRB assay are suitable to be used in clinic.②The drug-sensitivity of oral maxillofacial squamous ceil carcinoma is correlated to the drug. ③The effect of combination therapy is better than the single drug.