乙醇提取冷冻结晶去杂精制磷脂酰胆碱

Phospholipids purification with alcohol solution and removal impurity by refrigerating crystal

该文研究了在低温条件下,用乙醇作为溶剂,通过冷冻结晶去杂,从粗制大豆磷脂中精制磷脂酰胆碱的方法。通过单因素试验确定了影响磷脂酰胆碱纯度的相关因素。用响应面试验对精制条件进行了优化,结果表明冷冻时间、冷冻温度、液料比都对磷脂酰胆碱的纯度产生一定的影响。其中冷冻时间,冷冻温度的影响最为显著。经响应面分析优化得到了最佳工艺条件为:温度为-20℃、冷冻时间为23.56 h、液料比为8.73∶1(V/m)。在此条件下得到磷脂酰胆碱的预测纯度为70.73%。经过验证实验和高效液相法检测,实测在此条件下:磷脂酰胆碱的纯度为70.1%,得率为23.0%。中试结果为:磷脂酰胆碱的纯度为68.8%,得率为22.7%。表明此方法简单可行,适宜于工业化生产。

Method was introduced to refine phosphatidylcholine from rude soybean lecithin using alcohol and remove impurity by refrigerating crystal in the condition of low temperature. The related factors influencing the purity of phosphatidylcholine were studied through single-factor experiments. The response surface experiments were used to optimize the conditions of refinement. Results show that freezing time, temperature and the ratio of liquor to material have some influence on the purity of phosphatidylcholine. The significant factors are cooling time and freezing temperature. The optimization technology are obtained using the method of response surface experiments, the freezing time is 23.56 h, the freezing temperature is -20℃ and the ratio of liquor to material is 8.73 ： 1 （V/m）. In these conditions, the prediction purity of phosphatidylcholine is 70.73%. Through the verification test and High Performance Liquid Chromatography（HPLC） detection. The purity of phosphatidylcholine is 70.1% and the yield is 23.0%. Semi-works production confirmed the purity of phosphatidylcholine is 68.8% and the yield is 22.7 %. All the works prove this method can be used for industrial manufacture.