高效液相色谱同时测定祁连獐牙菜中3种有效成分的含量

Simultaneously Determination of Three Efective Components in Swertia przewalskii with HPLC

目的：建立反相高效液相色谱法同时测定祁连獐牙菜中3种有效成分含量的方法。方法：采用ZORBAX SB-C18（250mm×4．6mm，5μm）色谱柱，流动相甲醇和水（含0．04％磷酸）的比例在0—20min内由20：80至30：70梯度洗脱，流速1ml／min，检测波长240nm，柱温35℃。结果：落干酸、獐牙菜苦苷、龙胆苦苷与其他成分达到基线分离。线性范围分别为0，05～0．625μg（r=0．9999）、0．0095～2.9μg（r=0．9998）和0．0486～2．56μg（r=0．9996），加标回收率分别为103％（3．9％）、98，2％（4．0％）、101％（3，5％）。结论该方法简便、快速，结果准确、可靠。

The objective of the research was to establish a quantitative method of simultaneously determination of loganic acid, swertiamarin and gentiopicroside in Swertia przewalskii with RP-HPLC. The samples were separated in the column of Zorbax SB-C18（250 mm × 4.6 mm,5μm） ,which eluted with methanol and water（0.04% phosphoric acid）. The ration of methanol and water was increased from 20：80 to 30：70 in 20 min with a detected wavelength of 240 rim, the flowing rate of 1 ml/min,column temperature of 35 ℃. The result indicated three conpounds were isolated. The linear range of loganic acid, swertiamarin and gentiopicroside were 0.05 - 0.625 μg（ r -- 0.999 9） ,0.009 5 - 2.9（ r = 0.999 8）and 0.048 6 ～ 2.56 μg（ r = 0.999 6）, respectively. The average recoveries were 103%（3.9%）,98.2%（4.0%） and 101%（3.5%）,respectively.