毛细管气相色谱内标法测定防霉剂中丙酸

Determination of Propionic Acid in the Antimicrobial with Capillary Gas Chromatography by Using Internal Standard Method

建立了以巴豆酸为内标物,用毛细管气相色谱快速测定防霉剂中丙酸含量的方法.样品用乙酸乙酯提取,以DM-FFAP毛细管柱分离,火焰离子化检测器检测.结果表明,在0.5-5.0 mg/mL浓度范围内,丙酸和巴豆酸的质量浓度比与峰面积比的回归方程为y=0.0101+0.7774x,相关系数r=0.9996,检出限为5.0 mg/L;样品加标回收率(n=6)为91.7%-99.9%,相对标准偏差为3.09%.该方法简单、快速、准确,可用于测定防霉剂中的丙酸含量.

A capillary gas chromatographic method for determination of propionic acid in the antimicrobial was developed and butenoic acid was selected as the internal standard substance. The samples were first extracted with ethyl acetate and then analyzed by DM-FFAP capillary gas chromatography with FID. The propionic acid and butenoic acid were separated from each other within 3 min. The calibration curve was linear over a range from 0. 5 to 5.0 mg/mL with a correlation coefficient of 0. 9996 and the detection limit was 5. 0 mg/L. The recovery for spiked standards was between 91. 7% and 99. 9%（n=6）and the relative standard deviation was 3. 09%. The method presented the advantages of simple pretreatment and high precision. The method can be used to test the propionic acid in the antimicrobial.