摘要
目的研究氟桂利嗪(FNZ)、拉莫三嗪(LTG)及两药联合经孕鼠给药,对胎鼠宫内缺血缺氧性脑损害的保护作用。方法将孕20d Wistar大鼠40只,随机均分为FNZ组,LTG组,FNZ+LTG组和对照组。前3组大鼠于脑缺血缺氧术前3d开始灌胃给药,每天1次,分别为FNZ0.5mg/(kg.d),LTG10mg/(kg.d),FNZ0.5mg/(kg.d)+LTG10mg/(kg.d)。第3天给药后3h,每组取8只孕鼠制作宫内缺血缺氧模型,剩余2只孕鼠直接行剖宫产取胎鼠,每胎5只。于缺血缺氧后6、12、24、48h分别取胎鼠血清0.5mL,采用电化学发光法测定血清磷酸肌酸激酶脑型同工酶(CK-BB)水平。结果胎鼠缺血缺氧后,血清CK-BB水平明显升高。FNZ组、LTG组及FNZ+LTG组胎鼠缺血缺氧后各个时间点血清CK-BB较对照组明显降低,差异均有极显著意义(均P<0.01)。联合用药较单个药物应用效果更明显,组间比较差异亦有极显著意义(P<0.01)。结论产前预防性口服FNZ、LTG对胎宫内缺血缺氧性脑损伤具有保护作用,联合用药效果更明显。
Objective To investigate the neuroprotective effects of flunarizine(FNZ) and lamotrigine (LTG), and their combination on the hypoxic-ischemic brain damage in fetal rats. Methods Forty cases pregnant Wistar rats ( pregnancy for 20 days) were randomly assigned into FNZ group, LTG group, FNZ + LTG group and the control group. The drugs were administered through feeding tube 3 days before the hypoxic-ischemic operation once a day. The dosages in the three study groups were: FNZ 0.5 mg/(kg· d), LTG 10 mg/(kg· d), FNZ 0.5 mg/(kg· d) + LTG 10 mg/ (kg· d), respectively. Three hours after the third dose, 8 cases of pregnant rats from each group were operated on for the fetal rats hypoxic-ischemic brain damage model, 2 cases as the control, and 5 fetal rats were taken out from each pregnant rat. The serum (0.5 mL) was taken from the fetal rats 6, 12, 24, 48 h after the birth, respectively. The concentration of brain-specific creatine kinase (CK-BB) in serum was detected by the electrochemistry radiation method. Results The concentrations of serum CK-BB in hypoxic-ischemic fetal rats were significantly higher than those in the no ischemic group. The concentrations of serum CK-BB in FNZ group, LTG group, FNZ + LTG group were distinctively lower than those in the control group, there were significant differences among the four groups and between the combineddrug group and the single - drug group, P 〈 0.01. Conclusions Antenatal oral FNZ and LTG have positive neuroprotective effects on intrauterine hypoxic-ischemic brain damage. The effects of the combination of the two drugs are much better.
出处
《中国校医》
2007年第2期127-129,共3页
Chinese Journal of School Doctor
基金
深圳市科技局立项(200405204)