K_2S_2O_8-luminol化学发光体系检测银纳米粒子寡聚核苷酸探针研究

Determination of Oligonucleotides Modified Ag Nanoparticle Label with K_2S_2O_8-luminol Chemiluminescence System

实验将3’端修饰巯基的寡聚核苷酸标记在纳米银粒子上，并基于银纳米粒子溶解后产生的Ag^＋对K2S2O8-luminol化学发光体系的催化作用，对标记寡聚核苷酸的银纳米粒子进行了定量检测．探讨了各种实验条件对该体系的影响并建立了最佳分析条件．其线性范围为5×10^-9～1×10^-6／mL，检出限（3σ）为2．0×10^-9g／mL．该方法操作简便，灵敏度高，为进一步的DNA杂交分析提供了一种新的检测手段．

In this contribution, 3 ＇-alkythiol-capped oligonucleotides were successfully attached to the Ag nanoparticles. Based on the catalytic action of Ag^＋ released from the nanoparticles on the chemiluminescence reaction of K2S2O8-luminol, the Ag nanoparticles modified with oligonucleotides were quantitatively determined. The experimental conditions were investigated systematically and the optimal conditions were established. There was a good linear relationship between the CL intensity and the concentration of Ag nanoparticles in the range of 5 × 10^- 9- 1 × 10^ -6 g/mL, and the detection limit （3σ） was 2.0 × 10^- 9 g/mL. The proposed method was sensitive and convenient, which may pave a new way for the detection of DNA hybridization.