激光显微切割技术在植物细胞和分子生物学研究中的应用进展

Review on Laser Microdissection Techniques for Plant Cell and Molecular Biology Research

激光显微切割技术是20世纪90年代后期发展,由现代物理学和生物学相结合的精确取材技术。该技术可以在显微镜下利用微激光束直接从不同的组织(包括活体组织)快速切割、分离和纯化生物体的目标组织、细胞及其组分,用于细胞和分子生物学的研究。笔者结合开展激光显微切割技术研究遇到的问题,从制片技术的改进、基因表达、DNA和蛋白质分析等方面,对激光显微切割技术在植物细胞和分子生物学研究中的应用进行了综述。

Laser microdissection provides a rapid isolating and purification modern method for biological tissues and cellular preparations. It directly cuts objects from heterogeneous tissues under the microscope. This review concerned an up-to-date advance in Laser microdissection techniques which are applied on to the plant cell and molecular biology researches in regard with the sample preparation, gene expression analysis, DNA and protein studies etc. Meanwhile, possible directions for future development of laser microdissection in plant research were put forward.