结合药效的刺五加纯化工艺优选

Pharmacodynamic Optimization of Purification Technique for Acanthopanax Senticosus Harms

目的优选刺五加水提物的纯化工艺。方法将刺五加水提物分别采用D-101大孔树脂、聚酰胺和正丁醇萃取工艺纯化,测定紫丁香苷和总黄酮含量,并比较3种纯化样品对模型大鼠缺血再灌注损伤和模型小鼠断头张口的抗脑缺血作用。结果3种纯化样品均具有抗脑缺血作用,其中D-101大孔树脂纯化样品的作用强于其它2种样品;紫丁香苷含量与刺五加抗脑缺血作用成正相关。结论D-101大孔树脂吸附法是刺五加提取物较佳的纯化工艺。

OBJECTIVE： To optimize the purification technique for the extract of Acanthopanax senticosus Harms. METHODS： The techniques of D- 101 macroporous resin adsorption, polyamide adsorption and n - butanol extraction were separately applied to purify the aqueous extract of Acanthopanax senticosus Harms. Syringin was detected by HPLC and total flavone was detected by UV. The anti - cerebral ischemia effects of three purified samples were compared using rat MCAO model and mouse decapitation gape model. RESULTS： The three purified samples all showed anti- cerebral ischemia effects, with the sample purified via D- 101 macroporous resin adsorption having stronger effects than the other two. The content of Syringin was correlated with anti - cerebral ischemia effects. CONCLUSIONS： D - 101 macroporous resin adsorption is a better technique for purifying the aqueous extract of Acanthopanax senticosus Harms.