酸水煮提法提取麻黄的工艺研究

Study on Acid-water Boiling Extraction Technique for Herba Ephedrae

目的优选麻黄中麻黄碱和伪麻黄碱的最佳提取工艺。方法用高效液相色谱法对麻黄中麻黄碱和伪麻黄碱进行含量测定;用正交试验法优选最佳提取工艺。结果最佳提取工艺为第1次用10倍量水,提取45min;第2次用8倍量水,提取30min;水的pH值为3。结论该提取工艺合理,含量测定方法简单、可靠。

OBJECTIVE： To optimize the technique for extraction of Ephedrine and Pseudoephedrine in Herba ephedrae. METHODS： HPLC was used to determine the contents of Ephedrine and Pseudoephedrine in Herha ephedrae, and orthogonal test was applied to optimize the extraction technique. RESULTS： The optimized extraction technique was as follows： using 10- fold water for the first time and extracting for 45 mins; using 8- fold water for the second time and extracting for 30 rains; setting pH of water at 3. CONCLUSIONS： This extraction technique is well- grounded, and the content determination method is simple, safe and reliable.