HPLC法测定注射用舒血宁中总黄酮醇苷的含量

Determination of Total Favonol Glycosides in Shuxuening for Injection by HPLC

目的：建立以高效液相色谱法测定注射用舒血宁中总黄酮醇苷含量的方法.方法：色谱柱为Dikma DiamonsilTM C18（200mm×4.6mm,5μm）,流动相为甲醇-0.5%磷酸溶液（54∶46）,流速为1.0mL·min^-1,检测波长为360nm,柱温为25℃.结果：总黄酮醇苷中槲皮素在0.007 1～0.085 2 mg·mL^-1（r=0.999 0,n=5）、山柰素在0.006 5～0.078 0 mg·mL^-1（r=0.999 7,n=5）、异鼠李素在0.001 5～0.018 0 mg·mL^-1（r=0.998 1,n=5）浓度范围内具有较好的线性关系;平均加样回收率总黄酮醇苷为101.2%（RSD=0.5%）,槲皮素为99.2%（RSD=0.9%）,山柰素为103.8%（RSD=0.8%）,异鼠李素为102.7%（RSD=2.0%）.结论：本方法测定结果准确、操作简便、灵敏度高、重现性好,可用于本品的质量控制.

OBJECTIVE： To establish an HPLC method for determination of the content of Total Favonol Glycosides in Shuxuening for Injection. METHODS： Dikma DiamonsilTM C18（200mm × 4.6mm, 5μm） column was used as chromatographic column, and methanol- 0.5% acid water （54 ： 46） as mobile phase. The detection wavelength was set at 360nm, flow rate at 1.0mL · min^-1, and column temperature at 25℃ . RESULTS： There was a good linear relationship when Quercetin of Total Favonol Glycosides was in the range of 0.007 1-0.085 2mg · mL ^-1（ r = 0.999 0, n = 5） ,Kaempferol in the range of 0.006 5-- 0.0780mg·mL^-1（r=0.9997, n =5）, and lsorhamnetin in the range of 0.001 5-0.0180mg·mL^-1（r=0.998 1, n =5） . The average recoveries of Total Favonol Glycosides, Quercetin, Kaempferol and Isorhamnetin were respectively 101.2% （RSD = 0.5% ）, 99.2% （RSD = 0.9% ）, 103.8% （RSD = 0.8% ）and 102.7% （RSD = 2.0% ） .CONCLUSIONS： This method is accurate in determination results, simple in operation, high in sensitivity and good in reproducibility, and can be used for the quality control of the preparation.