喉癌表皮生长因子受体表达的免疫组化定量分析

Quantitative immunohistochemical study of epidermal growth factor receptor(EGFR) in laryngeal polyps,dysplasia and carcinoma

运用免疫组化方法检测了喉癌、喉粘膜不典型增生、声带息肉组织各１８例中表皮生长因子受体（ＥＧＦＲ）的表达水平，结合显微图像分析仪进行定量分析。结果表明：在声带息肉组织中ＥＧＦＲ存在于基底细胞层，偶见于棘细胞层，而在喉粘膜不典型增生及喉癌组织的各层细胞均有表达。喉癌细胞中ＥＧＦＲ含量明显高于声带息肉及喉粘膜不典型增生细胞（均为Ｐ＜０．０１），病理分级不同的喉癌细胞ＥＧＦＲ含量不同，Ⅱ、Ⅲ级鳞状细胞癌中均较Ⅰ级中增高（Ｐ＜０．０５），喉粘膜不典型增生细胞较声带息肉基底层细胞ＥＧＦＲ含量无增高（Ｐ＞０．０５）。本结果提示：在喉部常见疾病中，ＥＧＦＲ的过量表达是喉鳞状细胞癌的特有表现，并且ＥＧＦＲ的表达水平同喉癌细胞分化程度有关。

Abstract The presence of EGFR was studied in patients with laryngeal squamous carcinoma,la-ryngeal dysplasia and vocal cord polyps. There were 18 cases in each group. The study was carried out on paraffin embedded sections by means of ABC technique ,employing a polyclone antibody of EGFR,and the quantitative analysis was made by RTIA(real time image anal-yser).Our results indicated that:1.The expression of EGFR was demonstrated in the basal cell layer and occasionaly in spinous cell layer of laryngeal polyps,while for laryngeal dyspla-sia and carcinoma,all cells showed positive staining.2. The expression of EGFR in laryngeal carcinoma was significantly increased in contrast to that in laryngeal dysplasia(P<0.01 ),and to that in laryngeal polyps(P<0. 01).The content of EGFR varied according to the pathological degrees,The EGFR in the stage 2 and 3 of laryngeal squamous carcinoma in-creased significantly in comparison with that of stage 1(P<0.05).3.There were no signifi-cant difference in the content of EGFR between laryngeal dysplasia cell and the basal cell of laryngeal polyps(P>0. 05).It is concluded that the overexpression of EGFR is the specific character of laryngeal carcinoma. The expression of EGFR is related to pathological differen-tiation the overexpression of EGFR is not theresult of proliferation but the presence ofmalignancy. The quantitative immunohis-tochemical study of EGFR provides a new objective method to the early and differential diagnosis of laryngeal carcinoma.