摘要
利用体外组织培养方法对11组喉鳞状细胞癌标本进行原代培养。结果原代培养平均时间2月,培养成功率63.6%(7/11),并可继续传代。介绍了组织块培养和胰蛋白酶消化分离培养两种培养方法以及去除成纤维细胞的方法。讨论了喉鳞癌原代培养中的注意事项。
Abstract Using tissue culture method in vitro,11 specimens of laryngeal squamous cell carcinoma were primarily cultured. The result showed that the average time for primary culture was 2 months,Of 11 specimens,7(63.6%)were successfully cultured and capable of continuing passage. This article introduced 2 kinds of culture methed and the method to get rid of fi-broblasts. Some key points of primary culture were also discussed.
出处
《耳鼻咽喉(头颈外科)》
1996年第6期369-371,共3页
Chinese Arch Otolaryngology-Head Neck Surg