小分子化合物J2对小鼠淋巴细胞的影响

Effect of Micromolecular Compound J2 on Murine Splenocytes

目的探讨小分子化合物J2对小鼠淋巴细胞的影响。方法24只BALB/c小鼠随机分为3组,并分别以空白液、CsA、J2、连续腹腔注射12d。取脾细胞分别给予ConA和抗CD3mAb刺激细胞增殖,采用ELISA法测定细胞上清中IL-2、IFN-γ、IL-4及IL-10等细胞因子的含量。比较各组细胞增殖指数及细胞因子含量。结果ConA刺激后J2组细胞增殖指数较对照组降低,分别为空白组(9.61±2.20),J2组(6.53±3.21);J2组IL-2和IFN-γ的含量较对照组低,两组差异均有统计学意义。CsA组与J2组比较差异均无统计学意义。抗CD3mAb刺激后J2组细胞增殖指数较对照组降低,分别为空白组(9.52±2.44),J2组(3.27±1.33);J2组IL-2和IFN-γ的含量较对照组低,两组差异均有统计学意义。CsA组与J2组比较差异均无统计学意义。各组IL-4及IL-10的含量差异无统计学意义。结论J2有抑制T细胞活化,减少Th1细胞因子分泌的作用。

Aim To investigate the effects of J2 on murine splenocytes. Methods 24 BALB/c were divided into 3 groups randomly and were treated with intraperitoneal injection of placebo, CsA and J2, respectively. The drugs were delivered for 12 days consecutively. Murine splenocytes were stimulated by ConA and anti-CD3mAb. IL-2, IFN-γ, IL-4 and IL-10 of cyto- supernatant were measured by ELISA. The index of cell proliferation and production of cytokine was compared. Results After stimulation by ConA, the average index of cell proliferation in the control group was （9.61 ±2.20）. Treatment with J2 led to a statistically significant decrease of index of cell proliferation （6.53 ± 3.21, P 〈 0. 01 ） and production of IL-2 and IFN-γ. But compared with treatment with CsA there was no statistically significant difference between the two groups （ P 〉0.05）. After stimulation by anti-CD3mAb, the average index of cell proliferation in the control group was （9.52 ± 2.44）. There was no statistically significant difference between the groups. Conclusion These results showed that J2 could inhibit proliferation of T ceils and decrease the production of cytokine by Th1.