不同诱导培养基对冬小麦组织培养效果的影响

Influences on the Effect of Triticum Aestivum Culture by Different Induction Medium

两种不同的诱导培养基诱导冬小麦的成熟种胚，用得到的愈伤组织进行分化，通过对愈伤组织成苗率、分化率、褐死率的统计分析，结果表明，各个品种用最好的诱导培养基得到的愈伤组织质量提高，成苗率、分化率都大于用基本的诱导培养基得到的愈伤组织；对两种愈伤组织同时进行愈龄实验，发现不同愈伤组织培养的最适分化时间不同，继代一次分化率、成苗率都最高；随着继代代数的增多，褐死率呈明显的上升趋势。

Through inducing the mature embryo of Triticum aestivum by two different induction media, using the gained callus to make differentiation, by statistically analyzing the frequency of green plantlet differentiation, frequency of callus differentiation, frequency of callus brown, getting the results： the callus quality of different varieties increased by using the best medium, and the frequency of green plantlet differentiation, frequency of callus differentiation were all higher than callus got from the basic medium. Carrying through the callus time experimentation of the two different callus at the same time, found that the best differentiation time of the two calluses was different, when subculture one time, the frequency of green plantlet differentiation, frequency of callus differentiation were all at the highest value. But with subculture more time, the frequency of callus brown shows obvious rising trend.