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亚甲基四氢叶酸还原酶在小鼠耐氨甲蝶呤细胞中的表达

Study on the Expresssion of MTHFR in MTX-resistant Mice Cell Lines
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摘要 目的在RNA水平研究小鼠耐氨甲蝶呤细胞中亚甲基四氢叶酸还原酶（MTHFR）的表达情况。方法采用RT-PCR的方法，对小鼠氨甲蝶呤（MTX）抗性细胞系中MTHFR的表达进行分析。结果在NIH3T3、3T3R500、MTX100、M3T200中MTHFR的表达量基本相同，而在MTX300中MTHFR的表达量明显下降。结论在RNA水平，耐药细胞耐药浓度的改变可导致MTHFR表达改变。 Objective To study the expresssions of MTHFR in Methotrexate-resistant mouse cell lines. Methods The expression of MTHFR is detected by RT-PCR in the MTX-Resistant mouse cell lines. Results As concentration differs in NIH3T3 and MTX-resistant mouse cell lines, the expressions of MTHFR are different. In NIH3T3,3T3R500,MTX100 .MTX200, the expressions of MTHFR RNA are approximately the same, but it is markedly lowered in MTX300. Conclusion The expression of MTHFR RNA way be changed by high level of MTX.
作者 李丁 刘明睿 王哲 赵育桢 白静 傅松滨
机构地区 哈尔滨医科大学医学遗传学研究室 黑龙江省生物医药工程重点实验室
出处 《国际遗传学杂志》 CAS 2007年第2期92-94,F0004,共4页 International Journal of Genetics
基金 国家自然科学基金（30370783） 教育部高等学校博士学科点专项科研基金（20040226001） 黑龙江省教育厅振兴老工业基地重大科技项目 黑龙江省教育厅科研项目
关键词 MTHFR RT—PCR MTX MTHFR RT-PCR MTX
分类号 R [医药卫生]
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参考文献18

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国际遗传学杂志
2007年 第2期

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