摘要
目的观察灯盏花素对大鼠脑缺血再灌后海马CA1区神经元内游离钙离子浓度的影响。方法采用钙离子成像系统检测海马CA1区神经元内钙离子浓度。实验分为(1)正常组:大鼠不做任何处理,直接断头取脑分离出神经元,观察神经元内钙离子浓度;(2)再灌注组:制作大鼠的全脑缺血模型,并以再灌后分1.5、3.0、4.5、6.0 h 4个时间点进行观察;(3)灯盏花素组:在制作全脑缺血模型前15 min腹腔注射灯盏花素50 mg/kg,并以再灌后分1.5、3.0、4.5、6.0 h 4个时间点进行观察。每组(时间点)均检测11个神经元。结果再灌注组再灌后1.5、3.0、4.5 h时间点与正常组比较,Ca2+浓度升高(P<0.05或P<0.01);再灌后6.0 h神经元内游离Ca2+浓度与正常组比较,差异无统计学意义。灯盏花素组再灌后1.5、4.5 h时间点Ca2+浓度比再灌组相同时间点低(P<0.01);而再灌后3.0、6.0 h Ca2+浓度与再灌组相应时间点比较,差异无统计学意义。结论全脑缺血前给予灯盏花素可降低全脑缺血再灌后大鼠海马CA1区神经元内游离Ca2+浓度。
Objective To observe the effect of breviscapine on the intracdlular calcium concentration ([ Ca^2+ ]i) of the hippocampal neurons in rats with pantobrain ischemia-reperfusion. Methods The pantobrain ischemia rat model was made. A breviscapine treated group,a reperfusion group and a normal control group were set up. At 1.5 h, 3.0h, 4.5 h and 6.0h in the reperfusion period, the hippocampal CA1 neurons were dissociated acutely and dyed with Fura-2/AM. The [Ca^2+ ]i of single hippccampal neuron was measured with the Tillvision system for judging the effect of breviscapine. Results The [Ca^2+ ]i of single hippocampal neuron were increasing from reperfusion time of 1.5 h to 4.5 h in the breviscapine treated group compared with that of the normal control group (P〈0.05), without significantly difference at time point of 6h. The [Ca^2+]i concentration of the hippocampal CA1 neurons of the breviscapine treated group were higher than that of the reperfusion group at time points of 1.5 h and 4.5 h, P 〈 0.01, but not at time points of 3.0 and 6.0 h. Conclusion Breviscapine might have a protective effect on the cerebral ischemia-reperfusion injury through decreasing the [Ca^2 +]i and calcium overloading of the hippocampal neurons.
出处
《广东医学院学报》
2007年第1期7-8,14,共3页
Journal of Guangdong Medical College
基金
广东省中医药管理局资助课题(1060100)