妊娠期高血压疾病患者胎盘缺氧诱导因子-1α与脐血瘦素的关系及临床意义

The expression of hypoxia inducible factor-1alpha (HIF-1α) in the placenta of pregnancy induced hypertension and its relation to the leptin in umbilical vein blood

目的:通过对胎盘缺氧诱导因子-1α及脐静脉血瘦素水平的检测,探讨两者之间的关系,以及两因子对妊娠和妊娠期高血压疾病的影响。方法:随机选取住院病人65例,其中正常孕足月孕妇10例(正常组),妊娠期高血压疾病55例(高血压组)。高血压组中包括妊娠期高血压15例、轻度子痫前期15例、重度子痫前期15例、子痫患者10例。用免疫组化法检测胎盘组织中的缺氧诱导因子-1α,用放射免疫法检测脐静脉血的瘦素水平。用彩色病理图像处理软件将免疫组化结果进行平均光密度量化,数据用SPSS10.0统计软件分析。结果:①高血压组胎盘缺氧诱导因子-1α及脐静脉血瘦素水平较正常组增高,差异有显著性(P<0.01)。②高血压组胎盘缺氧诱导因子-1α与脐静脉血瘦素水平、新生儿体重呈正相关(P<0.01),而正常组相关性不明显(P>0.01)。结论:缺氧诱导因子-1α在妊娠期高血压疾病中,对胎儿的发育有促进作用,并对胎盘瘦素的分泌有影响。

Objective： To find the relation between HIF - letand leptin through detecting them in placenta and umbilical vein blood, and the effect on the disease. Methods： Sixty -five patients selected in hospital at random were divided into five groups. Ten cases were included in normal group, fifteen in gestational hypertension, fifteen in mild pre -eclampsia, fifteen in severe pre -eclampsia, and ten in eclampsia. Immunohistchemistry was used to measure HIF - 1 α, radioimmunoassay was used to determine leptin. The mean optical densi- ties （MOD） of HIF - 1 etstained by DAB were analyzed by high pathological image analysis system （HPIAS）. Statistical data were analyzed by SPSS 10. 0. Results： ①HIF - letpositive expression rate and leptin level in hypertension groups were higher than that in normal group （P 〈0. 01 ） . ②There were positive correlations between HIF -letand leptin, HIF -let and newborn weight in hypertension group, but it had no means in normal group. Condusion： HIF - 1 α improves the development of infant and significantly affects the secretion of leptin.