高效抗逆转录病毒治疗对AIDS患者MCP-1和MSP水平的影响

Impact of highly active antiretroviral therapy on plasma MCP-1 and MSP in AIDS patients

目的:研究高效抗逆转录病毒治疗(HAART)对AIDS患者血清中MCP-1、MSP水平的影响。方法:采用ELISA法检测40例经HAART3个月的国内AIDS患者及84例经HAART3至6年的德国患者血清MCP-1、MSP的水平,荧光定量PCR测定HIV-RNA滴度,流式细胞术检测CD4+T细胞计数。结果:HIV-1感染者血清MCP-1的水平显著高于正常人群(P<0.01),MSP则显著低于正常人群(P<0.01);经短期HAART(3月)后,MCP-1水平下降(P<0.01),MSP无明显改变;长期HAART(3至6年)后MCP-1水平显著升高(P<0.01),而MSP显著下降(P<0.01),MCP-1和MSP水平的变化呈负相关(r=-0.99,P=0.029)。结论:MCP-1、MSP与AIDS病程进展有密切关系,HAART对AIDS患者MCP-1、MSP水平有显著的改变,进而改善患者病程进展。

Objective： To study the effect of highly active antiretroviral therapy （HAART） on plasma levels of MSP and MCP-1 in AIDS patients. Methods： Forty Chinese AIDS patients were treated with HAART for 3 months and 84 German AIDS patients with HAART for 3 to 6 years.The pre-treatment and post-treatment plasma levels of MSP and MCP-1 were measured by enzyme-linked immunosorbent assay （ELISA）,and their correlations with CD4＋ cell counts and viral loads were analyzed. Results： The mean levels of MCP-1 were significantly higher and MSP were significantly lower in HIV-infected patients compared with the HIV-negative controls （P〈 0. 01）. After HAART for three months,there were no significant changes in the levels of these cytokines. But after long-term HAART （for 3 to 6 y）,the level of MCP-1 was increased and that of MSP decreased significantly （P〈0. 01）. There was a negative correlation between MSP and MCP-1 levels,and the same for MSP level and CD4＋cell counts; while there was a positive correlation between MCP-1 levels and CD4＋ cell counts. Conclusion： The changed plasma levels of MSP and MCP-1 are associated with HIV-1 infection and HAART may reverse the levels of these two cytokines.