高效液相色谱法测定复方黄芩消炎片中黄芩苷的含量

Determination of Content of Baicalin in Fufang Huangqin Anti-inflammatory Tablet by HPLC

目的:建立用高效液相色谱法测定复方黄芩消炎片中黄芩苷含量的方法。方法:采用Lichrospher色谱柱(C185μm,4.60 mm×250 mm),以甲醇-水-磷酸(47∶53∶0.20)为流动相,流速为1.00 mL.min-1,检测波长为280 nm。结果:黄芩苷的线性范围为(0.40-2.41)μg(r=0.999 9),平均回收率(n=5)为97.19%(RSD=2.00%)。结论:方法准确、可靠、重复性好。

Objective： To establish a method for determining baicalin in Fufang Huangqin Anti-inflammatory Tablet by HPLC. Methods： Lichrospher C18 （4.60 mm × 250 mm, 5 μm）was used, the methanol-water-phosphoric acid （47：53：0.20） was served as mobile phase, and its flow rate was 1.0 mL/min, wavelength for determining was 280 nm. Results：The linearity range was 0.40 - 2.40 （r = 0.999 9）. The average recovery was 97.19%, RSD = 2.00. Conclusion： This method is simple, highly sensitive, and accurate. It may be used for the quantitative determination of Fufang Huangqin Anti-inflammatory Tablet.