超重力处理及盐胁迫对绿豆幼苗生理生化指标的影响研究

Effects of hypergravity and salt-stress on the physiological and biochemical indices of mung bean seedlings

试验对绿豆(Phaseolus radiatus)刚萌动种子进行不同超重力和不同时间处理,并用9g／kg NaCl溶液模拟盐胁迫。结果表明:随处理超重力加大,其发芽率(Gr)和发芽指数(Gi)降低;3000×g处理组的根系活力、游离脯氧酸含量、超氧化物歧化酶(SOD)活性显著高于2000×g处理,4000×g与3000×g处理间差异不显著(SOD除外);处理组丙二醛(MDA)含量均低于对照,3000×g比2000×g处理丙二醛含量显著减少,4000×g和3000×g处理间差异不显著;2000×g、3000×g超重力处理随处理时间的延长,根系活力、游离脯氨酸含量、SOD活性呈增加趋势,而4h处理与3h处理间差异不显著,MDA含量随处理时间的延长呈降低趋势。超重力处理可提高绿豆幼苗的抗盐性,以3000×g、4h处理为宜。

Mung bean（Phaseolus radiatus）seeds in germination were treated with different hypergravities for different times,and 9g/kg NaCl solution was used to imitate the salt stress on mung beans tested in the experiment. The results show that germination rate and germination index decrease with hypergravity increasing. The roots activity,content of free proline and activity of superoxide dismutase（SOD）of seedlings under 3000×g treatment are significantly higher than those under 2000×g, and the difference between 4000× g and 3000× g is not significant except SOD. The contents of malondialdehyde （MDA）of seedlings of all treatments are lower than that of the control, that of 3000× g treatment is lower significantly than that of 2000×g,those of 4000×g and 3000× g are not different significantly. Under 2000×g and 3000 × g hypergravity treatments,the roots activity, the content of free proline and SOD activity of mung bean seedlings increase with the time prolongation. The treatments of 4h and 3h are not different significantly, the content of MDA decreases with the time prolongation. It is concluded that the salt tolerance of mung bean seedlings is improved by hypergravity treatment of 3000× g for 4h.