染料木素的磷酰化结构改造及与溶菌酶的弱相互作用

Phosphorylated modification of genistein and their interaction with lysozyme

采用改进的Atherton-Todd反应对染料木素进行磷酰化结构改造,得到了4种新的磷酰化产物,并通过ESI-MS,NMR进行了结构确认,结果发现磷酰化试剂对染料木素羟基的磷酰化具有较好的选择性,反应主要发生在染料木素7位羟基;进一步利用ESI质谱技术,对4种磷酰化染料木素与大分子溶菌酶的弱相互作用进行测定,显示所有的磷酰化产物均与溶菌酶形成非共价复合物。

A series of genistein＇ s phosphates were synthesized through a simplified Atherton-Todd reaction and the structures of the phosphates were determined by electrospray ionization mass spectrometry （ESI-MS） and NMR. In case of monophosporyl derivatives, NMR spectra show that substitutions occur at the 7-position of genistein but not at its 4＇ and 5-position. Moreover, the non-covalent complexes of lysozyme with the four new genistein phosphates were detected by ESI-MS.