理气化痰祛瘀方对非酒精性脂肪性肝炎大鼠血清TNF-α CRP的影响

Effects of Regulating Qi to Dissipate Blood Stasis and Phlegm on Serum Levels of TNF-α and CRP in Rats with Nonalcoholic Steatohepatitis

目的:观察理气化痰祛瘀方对高脂饮食诱导的非酒精性脂肪性肝炎(NASH)大鼠血清肿瘤坏死因子(TNF-α)、C-反应蛋白(CRP)的影响,探讨其可能作用机制。方法:以高脂饮食喂养SD大鼠12周,建立大鼠NASH模型,用不同剂量的理气化痰祛瘀中药干预,检测肝组织匀浆TC、TG的含量和血清ALT、AST水平,常规HE染色观察肝组织的病理改变,放免法和免疫比浊法分别检测血清TNF-α和CRP的水平。结果:模型组大鼠肝组织出现明显的脂肪变和炎症细胞浸润,其肝组织匀浆中TC、TG的含量及血清ALT、AST、TNF-α、CRP水平均明显高于正常对照组,血清CRP浓度和肝脏炎症活动度计分呈明显的正相关;应用理气化痰祛瘀方干预可改善NASH大鼠炎症活动程度,同时血清ALT、AST、TNF-α、CRP水平也显著降低。结论:TNF-α、CRP参与高脂饮食诱导的大鼠非酒精性脂肪性肝炎的发生,理气化痰祛瘀中药能显著降低大鼠血清TNF-α、CRP水平,控制NASH的发展。

Objective： To explore the potential mechanisms of nonalcoholic steatohepatitis （NASH） and regulating Qi to dissipate blood stasis and phlegm treatment by observing serum levels of tumor necrosis factor alpha （ TNF-α ） and c - reactive protein （CRP） in NASH rat model. Methods ： The model of nonalcoholic steatohepatitis in SD rats were induced by a term of 12 - week high fat diet, and interfering by different doses of Chinese Herbs with regulating Qi to dissipate blood stasis and phlegm for 12 weeks, the pathological changes were observed with HE staining, hepatic TG,TC were measured, and the serum content of ALT, AST were measured too. the serum content of TNF -α was measured by radioimmunoa - ssay and the serum content of CRP was measured by immunoturbidimetrie assay. Results： serious steatosis of hepatocyte and inflammation were found in model group; The contents of hepatic TG and TC of NASH rats were significantly higher than those of the control group （ P 〈 0.01 ）. The levels of serum ALT, AST, TNF-α and CRP of NASH rats were higher than those of control rats （ P 〈 0.05 ）, Chinese medicine, especially the high dosage, could remarkably reduce the serum levels of ALT, AST, TNF-α and CRP in NASH rats （ P 〈0.01 ）. The therapy effects of Chinese medicine were better than that of essential. The levels of CRP were well correlated with the scores. Conclusion ： TNF -α and CRP is concerned with the pathogenesis of NASH model rats with high - fat diet. Regulating Qi to dissipate blood stasis and phlegm can significantly reduce the serum levels of ALT, AST and TNF -α, CRP. It can prevent the development of NASH.