痹痛方镇痛抗炎作用研究

The Study of the Anti-inflammatory and Analgesic effects of Bitongfang

目的:观察痹痛方镇痛抗炎方面药效学作用,探讨其治疗类风湿性关节炎的药理学基础。方法:镇痛实验用热板法与扭体法;抗炎实验采用毛细血管通透性实验、棉球肉芽肿实验及佐剂性关节炎模型;观察药物对内毒素引起的TNF-α产生的影响。结果:在热板法与扭体法实验中痹痛方能明显提高小鼠痛阈;具有抑制毛细血管通透性、减轻棉球肉芽肿质量;显著抑制大鼠佐剂性关节炎急性足爪肿胀,对继发性的足肿胀也有明显的抑制作用。并能明显减少内毒素引起的TNF-α产生。结论:痹痛方具有明显镇痛抗炎作用。镇痛作用机制可能通过外周与中枢两种机制介导;而免疫调节作用可能为其抗风湿炎症的机制之一。

Ojbective： To observe the anti- inflammatory and analgesic effects of Bitongfang, and explore the pharmacological basis of therapeutical application on rheumatoid arthritis. Methods ： Hot plate test and writhing test were used in Analgesic study; permeability test of capillary vessel, cotton ball induced granuloma test and adjuvant arthritis model were used to explore the anti - inflammatory effects of the formula, The LPS - induced TNF -α production was explored. Results ： Bitongfang reduced the times of to.ion caused by acetic acid and reinforced the pain threshold of mice. And the formula could significantly restrain the permeability of capillary vessel and reduce the weight of the granuloma. Bitongfang could significantly inhabit primary acute inflammatory swelling and secondary inflammatory reaction adjuvant arthritis model rats. The formula can suppressed TNF -α production in the LPS - stimulated rats. Conclusion： Bitongfang has significant anti - inflammatory and analgesic effects, The analgesic activity involved peripheral and central mechanism, The immunomodulatory feature is involved in the anti - inflammatory effects of the drug.