摘要
DNA甲基化(m5C)在基因表达调控过程中发挥重要作用,而甲基结合域蛋白(MBD)是能特异识别甲基化位点的反式作用因子。为了探讨小麦中MBD基因的结构与功能,以拟南芥MBD基因的EST为基础,通过电子克隆结合RT-PCR方法分离克隆了包含ORF的小麦甲基结合域蛋白基因TaMBD3。序列分析显示,TaMBD3具有典型的甲基结合域,其中包含了能与甲基化DNA相结合的保守氨基酸残基。组织表达特性分析表明,TaMBD3在幼穗和茎中的表达量高于其它组织器官。采用电子定位的方法,将TaMBD3基因初步定位在6AS1-0.35-0.65和C-6BL3-0.36两个区域。
The 5-methylcytosines（m^5C） play a critical role in epigenetic control, often being recognized by proteins containing a MBD. In order to provide an insight into molecular characteristics and putative epigenetic functions of MBD in wheat, a gene with complete open reading frame （ORF） designated as TαMBD3 was identified in wheat by in silico cloning approach and cloned by RT-PCR. Amino acid sequence analysis showed that TαMBD3 contained a conserved methyl-CpG binding domain, which might play a putative role in epigenetic mechanism. Expression pattern of TαMBD3 showed that it expressed at higher level in inflorescence and stem than other organs. TαMBD3 was proved to be locating on chromosome 6AS1-0.35-0.65 and C-6BL3-0.36 by in silico mapping.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2007年第2期197-200,302,共5页
Journal of Triticeae Crops
基金
国家自然科学基金项目(30300195)
关键词
小麦
MBD基因
表达
电子定位
Wheat （T. aestivum）
MBD gene
Expression
In silico mapping
