肝动脉化疗栓塞对原发性肝癌患者树突状细胞的影响

The effect of transcatheter arterial chemoembolization on dendritic cells in hepatocellular carcinoma

目的探讨原发性肝癌患者肝动脉化疗栓塞(TACE)前后肝癌组织和外周血液中树突状细胞(DCs)的变化及其意义。方法选取经TACE治疗后二期手术切除的原发性肝癌患者标本17例(TACE+手术组)和未经TACE单纯手术切除的原发性肝癌患者标本30例(单纯手术组),用免疫组织化学方法检测肝癌组织中的DCs含量。另选经TACE治疗的原发性肝癌患者37例,分别于治疗前、治疗后第1,7,15天采集周围静脉血,用流式细胞仪检测治疗前后外周血DCs的含量。结果TACE+手术组肝癌组织中DCs数量明显少于单纯手术组(P<0.05);经TACE治疗后第7,15天,患者外周血DCs数量明显少于治疗前(均为P<0.05)。结论TACE治疗后,患者肝癌组织和血液中DCs数量的减少可能是TACE后肝癌易复发、转移的重要原因之一。

Objective To investigate the changes and significance of dendritic cells （ DCs ） in hepatocellular carcinoma （HCC） tissue and peripheral blood after transcatheter arterial chemoembelization （TACE）. Methods Surgical specimens were obtained from 17 HCC patients who had be＇en treated by operation after TACE and 30 patients operated on without TACE. The infiltration of DCs in HCC tissue was measured by immunohistochemistry method. Peripheral blood was obtained from 37 patients with HCC treated by TACE. The blood was obtained on the day before TACE, the first day , the 7 th day and the 15 th day after TACE respectively. The counts of DCs in peripheral blood were quantified by flow cytometry. Results The infiltration of DCs in HCC tissue which had been treated by oparation after TACE was lower than that of the groap not treated by TACE （ P 〈 0. 05 ）. On the 7th and 15th day after TACE, the counts of DCs in peripheral blood decreased significantly （ P 〈 0.05 ）. Conclusions After TACE, the infiltration of DCs in HCC tissue decreased, and the counts of DCs in blood were also decreased. This could be one of the reasons why HCC can recurr and metastasize easily after TACE.