节杆菌菌株LZP08X的分离鉴定及其降解苯酚特性

Isolation and Identification A Strain of Arthrobacter sp. LZP08X and Its Phenol-degrading Characters

目的:从柳州市某钢铁公司焦化污水处理厂的活性污泥中,筛选分离出高效降解苯酚的菌株,对其进行分类鉴定和苯酚降解特性的研究。方法:采用苯酚为唯一碳源和能源的无机盐培养基,经过富集培养,平板分离,耐受性试验和苯酚降解能力测定。结果:获得一株能高效降解苯酚的菌株,命名为LZP08X,通过形态观察和生理生化特征分析,初步鉴定为节杆菌属(Ar-throbacter sp.)。该菌株降解苯酚的最适条件为:温度35℃,pH 9.0,装液量20%(v/v);其降解苯酚过程符合一级反应动力学方程,在苯酚初始浓度为400mg/L时,于12h内,降解率达99%,降解速率常数K值为0.39,半衰期为1.78h。结论:节杆菌属(Arthrobact-er sp.)菌株LZP08X苯酚降解能力较强,对该菌的继续研究,可使其在含酚工业废水处理的实际应用中起到重要作用。

Objectives： A strain was isolated from activated sludge of burnt wastewater treatment plant of sted corporation in Liuzhou. Then the strain was identified and its ability in degrading phenol was studied. Methods： The methods was using a synthetic medium with phenol as the sole source of carbon and energy, enrichment culture, plate cultivation, phenol tolerating test and phenol degrading raeasurement to screen a strain. Results： A strain named as LZP08X was isolated and identified as Arthrobader sp. according to morphdogical observation, physiological and biochemical test. The optimum temperature , pH and volume of medium for phenol degradation were 35℃, 9.0 and 20%（v/v）, respectively. The degrading reaction of LZP08X accorded with Moned first - order equation. When the strain was cultured in the medium containing 400mg/L phenol, the degradation rate reached 99% in 12 hours, and the biedegradefion rate constant and half lifes were 0.39 and 1.78h, respectively. Conclusion： Arthurobacter sp. LZP08X could degrade phenol effectively, so it is sure that the subsequent research will make LZP08X act an important role in phenolic industrial wastewater treatmemt.