棉花枯萎病菌(Fusarium oxysporum f. sp. Vasinfectum)突变体的诱发与鉴定

Inducing and Identification of the nit Mutants of Fusarium oxysporum f. sp. Vasinfectum in Cotton

通过对32个棉花枯萎病菌菌株进行的突变体诱发与鉴定实践及技术方法探索,进一步验证了可以采用KClO3培养基来诱发突变体,并利用各突变体对不同N源的利用能力差异而采用MM,MH,MA,MO2培养基对各突变体类型进行鉴定。实验结果显示绝大多数菌株(93.7%)在KClO3培养基上能诱发出突变体,但多数(85%)突变体类型不全,且不同菌株诱得突变体的难易不同,个别菌株很难诱发到突变体。各突变体的诱发率高低依次为nit1>nit3>nit8>nitM,与国内外相关报道结果基本一致。

Studies on inducing and identification mutants of the 32 isolates of Fusarium oxysporum in cotton verified that KClO3 media could be used to induce mutants. The experiment results indicated that nit mutants arise spontaneously in most isolates of the 32 isolates of Xinjiang, when isolates were cultured on a medium containing KClO3. The nit mutants could be distinguished based on differential growth on MM, MH, MA, MO2 media containing different nitrogenous compounds as the sole source of nitrogen. The results indicated that most isolates can easily recover different type＇s mutants, however, most of them defined certain type＇s nit mutants, a few strains couldn＇t induce any type＇s mutants, all sorts of nit mutants ＇ percentage are nit1 〉 nit3 〉 nit8 〉 nitM. This result shows there is no difference compared with that at home and abroad.