激素和蔗糖浓度对新农1号狗牙根出愈和分化的影响

Effect of Hormone and Sugar on Callus and Differentiation of Cynodon dactylon (L.) Pers cv. Xinnong No.1

以新农1号狗牙根成熟种子为外植体，通过种子划破处理、不同激素浓度组合对种子发芽及愈伤组织诱导，不同继代培养基对愈伤组织增殖和质量影响的研究，结果表明：与未切割的新农1号狗牙根种子相比，切割后的新农1号狗牙根种子发芽率和愈伤组织诱导率均有显著提高，种子发芽率平均提高21％，愈伤组织诱导率平均提高14％，出愈时间提早5～10d；愈伤组织诱导中，添加4．00mg／L 2，4-D＋1．00mg／L 6-BA的诱导培养基所形成的愈伤组织质量较优；继代改造培养基中添加6％蔗糖＋0．50mg／L 2，4-D有利于胚性愈伤组织的生长，其分化率达50％。

The seeds of Cynodon dactylon （L.） Pers cv. Xinnong No. 1 Bermudagrass were used as the explants,the influence of seed incision and different hormone concentration combination on seed germination and the callus induction were studied,and the effect of different regeneration medium on the quantity and quality of callus was analyzed. The result showed, the seed germination rate and callus induction rate of the incised seeds were obviously higher than CK,the germination rate averagely increased by 21% ,callus induction rate averagely increased by 14%. The time of callus emergence came 5-10 d earlier than usual. Among the treatments,the quality of callus formed by induced medium increased by 4.00 mg/L 2,4-D＋ 1.00 mg/L 6-BA was better than other treatment. Adding 6% sugar and 0.50 mg/L 2,4-D into regeneration medium was beneficial to the embryogenic callus growth, the regeneration rate reached 50%.