PP_(333)对唐菖蒲试管苗增殖和生根的影响

Effect of PP_(333) on Growth Proliferation and Rooting of Gladiolus hybridus in Tissue Culture

以唐菖蒲“超级玫瑰”(Rose supreme)的茎尖为试验材料,将MS+0.1 mg/L NAA+0.4 mg/L16-BA作为增殖培养基,1/2MS+1.5 mg/L IBA+0.01 mg/L NAA作为生根培养基,添加不同浓度PP333,结果表明:增殖培养基中添加1.0 mg/L PP333对唐菖蒲试管苗增殖生长有促进作用,减少超度含水态苗的发生;生根培养基中添加0.1 mg/L PP333对唐菖蒲试管苗生根壮苗有良好效应。

Added PP333 at different concentrations to proliferative medium and rooting medium when the test tube seedlings of gladiolus were cultured. The medium for proliferation is MS ＋ 0.1 mg/L NAA ＋ 0.4 mg/L 6- BA. The medium for rooting is 1/2MS ＋ 1.5 mg/L IBA ＋ 0.01 mg/L NAA. The results showed that 1.0 mg/ L PP333 improved the proliferation of shoots. Meanwhile, the suitable concentration of PP333 （ 1.0 mg/L） could decrease the number of hyperahydric shoots. 0.1 mg/L PP333 in the medium for rooting improved rooting and growth.