土壤产几丁质酶菌株的筛选鉴定及产酶条件

Isolation and Identification of a Chitinase-Producing Bacterium in Soil and Studies on the Chitinase Fermentation Condition

利用几丁质为碳源,从土壤中筛选出3株产几丁质酶菌株,其中酶活最高的为一株革兰氏阴性菌.对该菌株应用16S rDNA法进行鉴定,结果为嗜麦芽窄食单胞菌(Stenotrophomonas maltophilia).通过单因素优化法和均匀设计法实验,结果表明,以质量分数0.5%的胶体几丁质为碳源,质量分数1.0%的蛋白胨为氮源,及30℃和pH值为7.2,发酵60 h的条件是菌株的最合适产酶条件.此时,胞外几丁质酶酶活力最高.

Three strains with chitinase activity were isolated from soil. The strain with the maximum enzyme activity level was a Gram negative bacterium which was identified as Stenotrophomonas maltophilia chitinase strain by 16S rDNA method. The study on the synthetic conditions for the chitinase by single factor optimization method and uniform design method showed that the optimal synthesis conditions for chitinase was as follows： the strain was cultured for 60 hours in a basic medium （DH 7.2, 30 ℃） added by 0.5% chitin and 1.0% peptone.