rIL－2激活的小鼠粘附性LAK细胞抗瘤活性和扩增的研究

ENHANCED ANTITUMOUR ACTIVITY AND RAPID EXPASION OF PURIFIED ADHERENT LYMPHOKINE-ACTIVATED KILLER CELLS IN MICE

小鼠脾脏淋巴细胞在体外ｒＩＬ－２培养下，可粘附到塑料板或玻璃瓶上，在除去非粘附性淋巴细胞后，粘附性ＬＡＫ细胞（Ａ－ＬＡＫ）在ｒＩＬ－２存在的条件下，４天内可迅速扩增３６倍，与ＬＡＫ细胞抗瘤活性相比，Ａ－ＬＡＫ细胞抗瘤活性明显增强，培养２０天后其抗瘤活性仍可达３３％，从形态学分析，Ａ－ＬＡＫ细胞主要由大颗粒淋巴细胞组成。

During the incubation of murine spleen cells with rIL-2(4000u/ml) a small percentage of cells became adherent to the surface of plastic or glass tissue culture flasks. After removal of non-adherent lymphoid cells,Adherent Lymphokine-Activated Kill Cells(A-LAK cells) cultured in vitro with IL-2 could be efficiently expanded to 36 fold in 4 days. A-LAK cells demonstrated greater antitumour cytotoxicity than unseparated LAK cells and retained antitumour activity for up to 20 days(%Cytotoxicity,33%,E/T,25/1),morphological analysis of Giemsa-stained smears indicated that A-LAK cells had the morphology of LGL with abundant cytoplasm and prominent cytoplasmic granules.