摘要
小鼠脾脏淋巴细胞在体外rIL-2培养下,可粘附到塑料板或玻璃瓶上,在除去非粘附性淋巴细胞后,粘附性LAK细胞(A-LAK)在rIL-2存在的条件下,4天内可迅速扩增36倍,与LAK细胞抗瘤活性相比,A-LAK细胞抗瘤活性明显增强,培养20天后其抗瘤活性仍可达33%,从形态学分析,A-LAK细胞主要由大颗粒淋巴细胞组成。
During the incubation of murine spleen cells with rIL-2(4000u/ml) a small percentage of cells became adherent to the surface of plastic or glass tissue culture flasks. After removal of non-adherent lymphoid cells,Adherent Lymphokine-Activated Kill Cells(A-LAK cells) cultured in vitro with IL-2 could be efficiently expanded to 36 fold in 4 days. A-LAK cells demonstrated greater antitumour cytotoxicity than unseparated LAK cells and retained antitumour activity for up to 20 days(%Cytotoxicity,33%,E/T,25/1),morphological analysis of Giemsa-stained smears indicated that A-LAK cells had the morphology of LGL with abundant cytoplasm and prominent cytoplasmic granules.
出处
《南通医学院学报》
1996年第2期157-159,共3页
ACTA Academiae Medicinae Nantong