摘要
为提高碱性磷酸酶（ＡＬＰ）活性测定的灵敏度，对以４－甲基伞形酮磷酸酯（４－ＭＵＰ）为底物的荧光法进行了研究。结果ＡＬＰ的线性范围达１．７×１０－５～１．７×１０－８ＩＵ／ｍｌ，检出限为４．０×１０－８ＩＵ／ｍｌ；用本法测定了２０份人血清样品，误差为３．５％；并测定了免疫标记物人ＩｇＧ－ＡＬＰ活性，发现其活性与荧光强度也呈线性关系。研究表明，通过纯化４－ＭＵＰ，优化分析条件，其灵敏度和实用性都得到明显提高。
In order to improve the sensitivity of detection of alkaline phosphatase(ALP)activity,a fluorometric method based on 4-methylumbelliferyl phosphate(4-MUP)as substrate was investigated.The linear range of ALP was 1.7×10 -5 ～1.7×10 -8 IU/ml,and the detection limit for ALP was 4.0×10 -9 IU/ml.Twenty samples of human serum were used(the relative error was 3.5%).Human IgG-ALP was also investigated and its concentration was linear with fluorescence intensity too.The results showed that the sensitivity was greatly improved by puring 4-MUP and optimizing the test conditions.
出处
《南通医学院学报》
1996年第2期162-163,共2页
ACTA Academiae Medicinae Nantong
关键词
碱性磷酸酶
荧光法
免疫球蛋白
免疫标记物活性
Fluorometry
Alkaline phosphatase
4-methylumbelliferyl phosphate
Immunoassay
