P53与P21基因蛋白在脑星形细胞瘤的表达

P 53 PROTEIN AND RAS P 21 PROTEIN EXPRESSION IN ASTROCYTOMAS

用Ｐ５３、Ｐ２１单克隆抗体免疫组化法检测Ｐ５３、Ｐ２１基因蛋白在３２例脑星形细胞瘤的表达。结果，Ｐ５３呈核型表达，阳性率为６２．５％；Ｐ２１为浆型表达，阳性度为７８．１％；两者阳性率及表达强度均随肿瘤分级增加而增加，其中１８例共同表达阳性（占Ｐ５３的９０％，Ｐ２１的７２％）。表明致癌基因的激活常伴抑癌基因的失活，两者起协同作用；并且Ｐ５３多为弱阳性（＋），而Ｐ２１多为阳性及强阳性（～），两者在表达强度上有显著性差异（ｐ＜０．０５）。揭示致癌基因的激活在肿瘤发生发展中起着较抑癌基因交重要的作用。

Thirty-two cases of astrocytomas (grade Ⅰ～Ⅳ)were examined to observe the expression of P 53 and ras P 21 protein with immunohistochemical method (S-P).The results are as follows:P 53 protein xpression was located in the nuclei,the positive incidence of overexpression was 62.5%;ras P 21 protein expression was located in the cytoplasmic,the positive incidence of overexpression was 78.1%.18 cases gave coordendly positive expression for P 53 and ras P 21 protein.The positive incidence and strength of overexpression of P 53 and ras P 21 protein in astrocytomas were related to the grade of the tumor,but the overexpression strength of ras P 21 protein is stronger than that of P 53 protein(0.01<P<0.05).