高效液相色谱-电喷雾电离质谱法分析人参皂甙

Analysis of ginsenosides by HPLC ESJ/MS

利用反相高效液相色谱-电喷雾电离质谱(HPLC-ESI/MS)方法,分离分析了人参皂甙。液相色谱采用乙腈-水流动相进行梯度洗脱,质谱法利用电喷雾电离质谱(ESI/MS)研究了3种人参皂甙的一级质谱(主要给出分子量)和二级、三级质谱(提供碎片离子信息),并通过质谱图的差异对其结构进行了鉴别,建立了高效液相色谱-质谱联用法分析人参皂甙的方法。

A reversed - phase high pertormance liquid chromatography - electrospray ionization mass spectrometry method has been developed for the analysis of ginsenosides. A high performance liquid phase chromatography （HPLC） analysis was performed on a revesed -phase C18 eolume using mobile phase of aeetonitrile and water mixture under gradien elution. HPLC - ESI/MS （ gain molecular weight） and HPLC - ESL/MS^2 and MS^3 （fragment ion information） of three ginsenosides has been studied by electrospray ionization mass spectrometry method and it identified the structure of Ginsenosides through the different of mass spectra. Moreover a HPLC - ESI/MS method was established for the analysis of ginsenosides.