摘要
目的建立协日嘎四味汤胶囊定性定量方法。方法采用薄层色谱法对协日嘎四味汤胶囊中所含黄柏、栀子、姜黄、蒺藜进行定性鉴别研究。采用高效液相色谱法测定其盐酸小檗碱的含量,色谱柱kromatosil C18,流动相为乙腈-0.03 mol/L磷酸二氢钠-磷酸(35:65:0.08),紫外检测波长为265 nm,流速为1.0 mL/min。结果薄层色谱鉴别方法专属性强,斑点分离良好。协日嘎四味汤胶囊中盐酸小檗碱HPLC测定样品平均回收率为96.5%,RSD=1.6%(n=9)。结论所建立的方法可作为协日嘎四味汤胶囊的定性定量方法。
Objective To develop a method to control the quality of Xieriga Siweitang capsules. Methods TLC was used to identify Golden cypress, Gardenia, Curcuma, Caltrop in Xieriga Siweitang capsules, HPLC was used to assay the content of hydrochloric berberine in Xieriga Siweitang capsules, the chromatographic column was Kromatosil C18, the mobile phase was acetonitrile:0.03mol/Lsodium dihydrogenphosphate:phosphoric acid (35:65:0.08) and the detective wavelength was 265 nm, and the flow rate was 1.0 mL/min. Results The TLC identification was highly specific with the clear spots. The average recovery rate of hydrochloric berberine in the capsules was 96.5% with RSD of 1.6%(n=9). Conclusion The method may be used to identify the quality and quantity of Xieriga Siweitang capsules.
出处
《湖南中医药大学学报》
CAS
2007年第2期40-42,共3页
Journal of Hunan University of Chinese Medicine
基金
长沙市科技计划重点项目(K0630012-12)