全自动免疫组化染色仪与人工操作的比较

Comparison between the usage of immunohistochemistry autostainer and manual operation

目的:探讨Autostainer全自动免疫组化染色仪在免疫组化中的应用。方法:选用常用的AAT、CerBb-2、CK、HBcAg、ER、PR、Ki-67等30多种一抗,使用统一的二抗,每种待测一抗用2种方法进行免疫组化染色,对比2种方法的实验情况。结果:使用全自动免疫组化染色仪染色背景干净,无边缘效应,着色均匀,阳性定位准确,均优于人工操作。结论:使用全自动免疫组化染色仪进行免疫组化染色不仅自动化程度高,省时省力,而且重复好,标准化程度高,提供更准确、更科学的实验结果。

Objective To discuss the application o immunohistochemistry autostainer in immunohistochemistry. Methods More than 30 kinds of first antibodies of the Pathology Department, such as AAT, CerBb-2, CK, HBcAg, ER, PR, Ki-67, and unified second antibodies were selected. Immunohistochemistry autostainer and manual operation were applied to the staining of the antibodies, and then the results by the above methods were compared. Results The antibodies stained by immunohistochemistry autostainer, gifted with clear background, no edge effect, uniform staining and accurate positive results, were all better than those by manual operation from all aspects. Conclusion Immunohistochemistry autostainer is highly automatic, time-saving, manpower-saving, repeatable and highly standardized.