转人源胸腺肽α_1基因聚球藻7942的高密度培养

High-cell density cultivation of transgenic Synechococcus sp. PCC7942 with human thymosin α_l gene

应用响应面分析方法(Response Surface Method,RSM),对转人源胸腺肽α1基因聚球藻7942的培养基及培养条件进行优化,优化后摇瓶培养转化藻的比增殖速率(μ值)可达0.500d-1,10 L全自动光生物反应器中培养转化藻μ值可达0.979 d-1,分别比正交实验提高了33%和161.1%,建立高密度培养的数字模型预测实验结果,预测值与实验值之间的相对误差在±5.0%以内,为响应面方法在微藻细胞培养中的广泛应用奠定了基础。

The response surface method（RSM）was used to optimize the medium and culture conditions for transgenic Sy-nechococcus sp. PCC7942 with human thymosin α1 gene . The results were as follows： NaHCO3 36 mg/L, KNO3 1.3 g/L, initial pH 10.0, light intensity 1800 Ix. Under these conditions, the specific growth rate（μ） of the transgenic strain could reach 0. 500 d^-1 through shake-flask culture, when cultivated in 10L autophotobioreactor, the μcould increase to 0. 979 d^- 1 , with the maximal yield of 0. 299 g/ L· d. There was no reduction of the bioactivity of UB-Tα1 in these culture processes.