β-细辛醚对PC12细胞和乳鼠皮层神经细胞形态学及细胞活力的影响

Effects of β-asarone on Morphology and Cell Viability in PC12 Cells and Cultured Rat Cortical Neurons

目的:观察β-细辛醚对PC12细胞和乳鼠皮层神经细胞形态学及细胞活力的影响。方法:用免疫细胞化学方法检测皮层神经细胞特异性标志物NSE、GFAP的表达;在体外将不同浓度β-细辛醚与PC12细胞和皮层神经细胞各作用24 h,用相差显微镜观察细胞形态的改变,并用MTT比色法检测细胞活力的变化。结果:原代培养的乳鼠皮层神经细胞大多数NSE呈阳性表达,少量GFAP呈阳性表达;β-细辛醚与PC12细胞作用24 h,低浓度β-细辛醚(7.5、15、30、60μg/ml)有促增殖作用,高浓度β-细辛醚(120、140、2480μg/ml)则可抑制其增殖,且随药物浓度的增加而增强;β-细辛醚与皮层神经细胞作用24 h,7.5、15、30、60、120μg/mlβ-细辛醚对其细胞形态和活力无明显影响,240μg/mlβ-细辛醚对其有促增殖作用,而480μg/mlβ-细辛醚对其则有损伤作用。结论:β-细辛醚可能有抗肿瘤和保护神经细胞的作用。

Objective： To observe the effects of β-asarone on the morphology and cell viability in PC12 cells and cultured neonate rat cortical neurons. Methods： The cultured neonate rat cortical neurocytes were stained immunocytochemically with NSE, GFAP antibodies, respectively; Morphological changes were observed under phase contrast microscope after PC12 cells and cultured rat cortical neurons with β-asarone of different concentrationfor 24h in vitro, and the cell viability of PC12 and cortical neurons were examined by MTT assay. Results： Most of the cultured neonate rat cortical neurocytes were positively stained with NSE antibody, and positively with GFAP in a less degree; Treatment of PC12 cells with concentrations of 7. 5,15,30,60 μg/ml β-asarone for 24h could facilitate the proliferation in PC12 cells, 120,240,480μg/ml β-asarone could inhibit the proliferation inversely, and with the concentration of β-asarone increasing, the inhibition was enhanced; Treatment of cultured neonate rat cortical neurons with concentrations of 7.5,15,30, 60,120 μg/ml β-asarone for 24h, there were no visible effects on morphology and cell viability, 240 μg/ml β-asarone could facilitate the proliferation obviously, but 480 μg/ml β-asarone induced injury on neurons. Conclusion ： β-asarone maybe has anti-tumor and protective effects on cultured neurons.