扶正培本法对乳腺癌患者树突状细胞的影响研究

Effect of Fuzheng Peiben Therapy on Dendritic Cell in Patients with Breast Cancer

目的:从细胞、分子水平上初步探讨中医扶正培本法对机体免疫功能的损害的修复作用及其机理。方法:用流式细胞术检测乳腺癌患者癌组织、引流淋巴结中CD83、CD80、CD86蛋白表达水平及在化疗前、后的改变。结果:乳腺癌患者癌组织、腋窝淋巴结细胞CD83、CD80、CD86蛋白与良性对照组比较明显降低(P<0.05或0.01),化疗前、后对比癌组织、腋窝淋巴结细胞CD83、CD80、CD86水平明显降低(P<0.05或0.01)。加中医扶正培本治疗后,化疗前、后对比癌组织、腋窝淋巴结细胞CD83、CD80、CD86水平没有显著性差异(P>0.05)。结论:肿瘤本身、化疗对机体免疫功能产生不良影响,扶正培本法可能参与激活树突状细胞功能,上调共刺激分子水平,帮助修复机体的免疫损伤。

Objective： To investigate the repairing mechanism of Fuzheng Peiben Therapy on cellular immunofunction in patients with breast cancer on cellar and molecular level. Methods： The tumor tissue and axillary lymph node samples were evaluated with the flow cytometry for CD83 ,CD80 ,CD86 before and after neoadujvant therapy. Results： The levels of CD83 ,CD80 ,CD86 were singificantly decreased in group A （ treaied with surgery only）, B （ treated with neoadujvant chemotherapy plus surgery） and C （ treated with neoadujvant chemotherapy plus surgery with shenqi Fuzheng Injection） compared with that in normal team（ P 〈 0. 05 or 0. 01 ） ;The levels of CD83, CD80, CD86 were significantly decreased in group A, B and C compared between before and after neoadujvant chemotherapy（ P 〈0. 05 or0. 01 ） ; They were not significantly decreased between group A and group C（P 〉0. 05）. Conclusion： The cellular immunity was inpaired considerably by neoadujvant chemotherapy. Neoadjuvant chemotherapy combined with Shenqi Fuzheng Injection can enhance the cellular immunity considerably.